Rat liver glutathione S-transferases. A study of the structure of the basic YbYb-containing enzymes.
نویسنده
چکیده
A purification scheme was devised that resulted in the resolution of a number of basic glutathione S-transferases from rat liver, three of which contained two subunits of molecular mass 23500 Da (i.e. Yb monomers). These were identified as transferases D, C and A by their elution positions from CM-cellulose and their specific activities towards a variety of substrates. Hybridization, immunotitration and peptide 'mapping' experiments demonstrated that transferases D, C and A comprise Yb2Yb2, Yb1Yb2 and Yb1Yb1 subunits.
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ورودعنوان ژورنال:
- The Biochemical journal
دوره 213 3 شماره
صفحات -
تاریخ انتشار 1983